The isolation and expansion of human cardiac stem cells offer new therapies for myocardial regeneration and repair.Cells could be expanded in vitro, providing a large number of human cardiac progenitor cells (hCPCs) for therapeutic applications. Nevertheless, despite significant advances and promising findings in animals, several issues have not yet been thoroughly investigated.First, an efficient approach is needed to isolate hCPCs from the human heart. Current methods are derived mostly from rodents, with only variable efficiency, thereby limiting the potential clinical application of hCPCs.Second, although hCPCs can be isolated from different sections of the heart, such as the septum and atrial appendages, correlation between the source of the sample and the number of hCPCs has not yet been determined. Finally, although it has been reported that the number of hCPCs increases during heart disease, it is unclear whether hCPCs could be isolated and grown from all patients.
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